Agenda - Introduction to NGS: Data Analysis Email Alerts

Agenda at a Glance

Here's an overview of what's happening at the event!

Day 1

Welcome and Course Overview
9:00 AM - 9:30 AM
Illumina Sequencing workflow
Introducing the workflow as foundational knowledge for data analysis
9:30 AM - 10:15AM
Break
10:15 AM - 10:30 AM
Sequencing run evaluation workshop
Learn to use Sequence Analysis Viewer (SAV) to troubleshoot common run errors
10:30 AM - 12:30 PM
Lunch
12:30 PM - 1:30 PM
Sequencing run evaluation workshop continued
Reviewing data from patterned flow cells
1:30 PM - 2:30 PM
Self-Study
Inter-session activities will be assigned to you to revise the content from day 1
2:30 PM - 5:00 PM

Day 2

Review of Day 1
9:00 AM - 9:15 AM
FASTQ Generation
What are FASTQs and how are they generated. Learn how to create a samplesheet using BaseSpace Sequence Hub (BSSH)
9:15 AM - 10:15 AM
Break
10:15 AM - 10:30 AM
FASTQ Troubleshooting
Learn why samples may end up in an 'Undetermined' FASTQ file and apply during troubleshooting scenario
10:30 AM - 11:15 AM
FASTQ Evaluation and Manipulation
Introduction FASTQC tools and how to manipulate FASTQs to enhance downstream analysis
11:15 AM - 12:00 PM
Lunch
12:00 PM - 1:00 PM
Secondary Analysis Quality Control
Learn about the important metrics to review during QC of secondary analysis data

Day 3

Review of Day 2
9:00 AM - 9:15 AM
DRAGEN Germline: Secondary Analysis
Learn about the DRAGEN Germline Application filters and reports
9:15AM - 10:30 AM
DRAGEN Germline Application Launch
Learn to launch the App using BSSH, ensuring all filters are considered carefully
10:30 AM - 11:30 AM
Break
11.30 AM - 11:45 AM
Introduction to Tertiary Analysis
Overview of Tertiary Analysis and Interpretation options
11.45 AM - 12:45 PM
Wrap up
12:45 PM - 1:00 PM